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SOLVED: Step 1: Once the CFU is determined, you can calculate the total amount of DNA in pg: DNA ug Concentration in ug/uL divided by volume in pL. In the lab, we used 10 μL of plasmid DNA at a concentration of 0.005 pg/μL.Step 2: You will not need to determine the fraction of the DNA that was spread on the plates. This is the volume of the solution you spread on the plates divided by the total volume in the tube. Because the units cancel, this is a unitless number.Step 3: Calculating the DNA
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