The CellPhoneDB 55, 56 Python package (v.3 .0) was used to infer putative cell–cell interactions. The scRNA-seq dataset was split by gut region and cell types with <30 cells in a given region were ...
Nascent proteins were labelled using a Click-iT HPG Alexa Fluor 488 Protein Synthesis Assay kit (Thermo Fisher, C10428). Cells were incubated with 50 μM HPG (Thermo Fisher, C10186) in T cell medium ...
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