STED microscopy is an important technique that can overcome the diffraction limit and resolve structures on the order of ~40mm. It is a powerful method that is particularly useful in a biological ...

Then, using Time-gated STED Microscopy, the research team made still images at 60 nanometre resolution (about one thousandth of the width of a human hair), as well as time-lapse image sequences ...

Stimulated emission depletion (STED) microscopy is one of the family of super-resolution techniques developed to allow imaging beyond the normal diffraction limit of optical microscopy. The principle ...

Nanoscopy describes the ability to see beyond the generally accepted optical limit of 200–300 nm. Stimulated emission depletion (STED) microscopy, developed by Stefan W. Hell and Jan Wichmann in 1994, ...

Light--and all waves--can bend around the corners of obstacles found along its path. Because of this phenomenon, called diffraction, it is impossible to focus light onto a spot that is smaller than ...

Researchers enhanced Stimulated Emission Depletion (STED) microscopy by replacing traditional fluorophores with nanographenes, enabling the observation of longer-duration processes, overcoming a ...

Both images show individual red blood cells. The one on the right, produced by STED, more clearly shows the Piezo1 proteins (in green), which are a mechanical calcium channel and play a crucial role ...

Novel developments also focus on quick and high-speed AFM to improve temporal resolution. 2 The combination of AFM with optical microscopy has expanded the number of possible applications, ...

A decade ago, the Nobel Prize in Chemistry was awarded to a trio of researchers for the development of super-resolved fluorescence microscopy. The announcement at the time stated that the researchers’ ...